Results available in ANY-maze

Section 1

Information measures

ANY‑maze includes the following information measures for each test. For full details about each measure see A detailed description of the ANY‑maze measures.

• Test number
• Animal number
• Treatment
• Treatment code
• Stage
• Trial number
• Apparatus
• The reason for test end
• Test date
• Test day of the week
• Test time
• Test time of day
• User
• Test notes
• Animal notes
• Animal lighter/darker than apparatus
• Animal length
• Percentage of frames tracked
• Percentage of tracked frames head tracked
• Source video file
• Recorded video file
• Video time when test started
• Time period
• Segment of test
• Location of movable zones

Section 2

Apparatus measures

ANY‑maze will score the following measures for the apparatus as a whole. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Test duration
• Time not hidden
• Total distance travelled
• Total distance travelled by the animal’s head
• First zone entered
• Visited zone list
• Investigated zone list

Speed measures

• Average speed
• Average speed when not hidden
• Maximum speed

Freezing measures

• Total freezing episodes
• Total time freezing
• Latency to start of first freezing episode
• Average freezing score

Mobility measures

• Total time mobile
• Total time immobile
• Total mobile episodes
• Total immobile episodes
• Latency to start of first mobile episode
• Latency to start of first immobility episode
• Latency to start of last mobile episode
• Latency to start of last immobility episode

Activity measures

• Total time active
• Total time inactive
• Total active episodes
• Total inactive episodes
• Longest active episode
• Shortest active episode
• Longest inactive episode
• Shortest inactive episode

Turning and rotation measures

• Rotations of the animal’s body
• Clockwise rotations of the animal’s body
• Anti-clockwise rotations of the animal’s body
• Absolute turn angle
• Absolute head turn angle
• Clockwise head turn angle
• Anti clockwise head turn angle

Rearing measures

• Number of rears
• Total time rearing
• Latency to first rear
• Average duration of a rear
• Maximum duration of a rear
• Minimum duration of a rear

Miscellaneous measures

• Path efficiency
• Number of line crossings
• On/off inputs positive reversal
• On/off inputs negative reversal
• Tracking quality
• Number of centre positions recorded
• Number of head positions recorded

RAPC measures

• RAPC – Type 1 errors
• RAPC – Type 2 errors
• RAPC – Door sequence

Section 3

Zone measures

ANY‑maze will score the following measures for a zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

Note that all key, on/off input, rotary encoder, signal, sensor, output switch, speaker, shocker, light controller, optogenetic laser controller, pellet dispenser and virtual switch measures are also reported separately for each defined zone.

• Number of entries to the zone
• Number of exits from the zone
• Number of entries of the animal’s head into the zone
• Time in the zone
• Time the animal’s head was in the zone
• Was first zone entered
• Number of bouts of investigation of the zone
• Total time investigating the zone
• Was first zone investigated

Zone investigation measures

• Longest bout of investigation of the zone
• Shortest bout of investigation of the zone
• Average duration of investigation of the zone
• List of the duration of each investigation of the zone

Hidden zone partial exit measure

• Number of partial exits from the zone
• Time partially exited from the zone

Distance measures

• Distance travelled in the zone
• Distance travelled until first entry into the zone
• Distance travelled by the animal’s head in the zone
• Distance travelled while investigating the zone
• Distance travelled before first investigation of the zone

Latency measures

• Latency to first entry to the zone
• Latency to first exit from the zone
• Latency to last entry to the zone
• Latency to the first entry of the animal’s head into the zone
• Latency to the first exit of the animal’s head from the zone
• Latency to first investigation of the zone
• Latency to end of first investigation of the zone

Speed measures

• Average speed in the zone
• Maximum speed in the zone
• Average speed while investigating the zone

Zone visit measures

• Longest visit to the zone
• Shortest visit to the zone
• Average duration of visit to the zone
• List of the duration of each visit to the zone

Mobility measures

• Time mobile in the zone
• Time immobile in the zone
• Immobile episodes in the zone
• Time mobile while investigating the zone
• Time immobile while investigating the zone
• Immobile episodes while investigating the zone

Activity measures

• Time active in the zone
• Time inactive in the zone
• Inactive episodes in the zone

Distance to the zone (when outside the zone) measures

• Initial distance from the zone
• Average distance from the zone
• Maximum distance from the zone
• Minimum distance from the zone
• Cumulative distance from the zone
• Average distance of the animal’s head from the zone
• Maximum distance of the animal’s head from the zone
• Minimum distance of the animal’s head from the zone

Distance to the zone border (when inside the zone) measures

• Average distance to the zone border
• Maximum distance to the zone border
• Minimum distance to the zone border
• Average distance from the animal’s head to the zone border
• Maximum distance from the animal’s head to the zone border
• Minimum distance from the animal’s head to the zone border

Movement relative to zone measures

• Time getting closer to the zone
• Time getting further away from the zone

Heading/orientation measures

• Initial heading error to the zone
• Signed initial heading error to the zone
• Average absolute heading error to the zone
• Time moving towards the zone
• Time moving away from the zone
• Time oriented towards the zone
• Time oriented towards the centre of zone when inside zone

Turning measures

• Absolute turn angle while in the zone
• Absolute head turn angle while in the zone

Freezing measures

• Freezing bouts in the zone
• Time freezing in the zone
• Freezing bouts while investigating the zone
• Time freezing while investigating the zone

Rearing measures

• Number of rears in the zone
• Total time rearing in the zone
• Latency to first rear in the zone
• Average duration of a rear in the zone
• Maximum duration of a rear in the zone
• Minimum duration of a rear in the zone

Whishaw’s corridor measures

• Time spent in Whishaw’s Corridor
• Distance travelled in Whishaw’s Corridor

Miscellaneous measures

• Time the animal’s head was in the zone when its centre was outside the zone
• Path efficiency to first entry to the zone
• Corrected integrated path length
• Number of line crossings while in the zone

Section 4

Point measures

ANY‑maze will score the following measures for a point. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Average distance from the point
• Maximum distance from the point
• Minimum distance from the point
• Average distance of the animal’s head from the point
• Maximum distance of the animal’s head from the point
• Minimum distance of the animal’s head from the point

Movement relative to point measures

• Time moving towards the point
• Time moving away from the point
• Time the animal’s head was moving towards the point
• Time the animal’s head was moving away from the point
• Average speed moving towards the point

Heading/orientation measures

• Initial heading error to the point
• Average absolute heading error to the point
• Time the animal’s head was oriented towards the point
• Time the animal’s head was oriented away from the point
• Number of times the animal’s head was oriented towards the point

Location of point measures

• Point X coordinate
• Point Y coordinate
• Approximate time at the point

Section 5

Sequence measures

ANY‑maze will score the following measures for a sequence. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of sequences
• Total time performing sequences
• Latency to start of first sequence
• Latency to completion of first sequence
• Average time to complete the sequence
• Maximum duration of a sequence
• Minimum duration of a sequence
• Total distance travelled during sequences
• Average distance travelled per sequence
• Maximum distance travelled during a sequence
• Minimum distance travelled during a sequence
• Average speed during the sequence

Section 6

Key measures

ANY‑maze will score the following measures for a key. Each measure is available for the apparatus as a whole (i.e. irrespective of where the behaviour occurred) and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of presses
• Time pressed
• Latency to first press
• Latency to first release
• Distance travelled before 1st press
• Longest press
• Shortest press
• Average press duration
• Press frequency
• List of activation durations

Section 7

On/off input measures

ANY‑maze will score the following measures for an on/off input. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the input activation occurred) and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 8

Rotary encoder measures

ANY‑maze will score the following measures for a rotary encoder. Note that each measure is available for the apparatus as whole, and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of rotations
• Time turning
• Distance
• Number of clockwise rotations
• Number of anti-clockwise rotations
• Number of reversals
• Number of half rotations
• Number of quarter rotations
• Degrees of clockwise rotation
• Degrees of anti-clockwise rotation
• Maximum RPM
• Minimum RPM
• Average RPM
• Average RPM while turning

Section 9

Signal measures

ANY‑maze will score the following measures for a signal or a filtered signal. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the input activation occurred) and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Average
• Maximum
• Minimum
• Time of maximum
• Time of minimum
• Baseline
• Baseline deviation
• Baseline standard deviation
• Time of the end of the baseline period
• Time of first positive deviation from baseline
• Time of first return to baseline from positive deviation
• Time of first negative deviation from baseline
• Time of first return to baseline from negative deviation
• Integral above baseline
• Integral below baseline
• Average maximum for each visit to zone
• Average time to maximum for each visit to zone
• Average minimum for each visit to zone
• Average time to minimum for each visit to zone
• Average at zone entry
• Average at zone exit

Section 10

Sensor measures

ANY‑maze will score the following measures for a sensor. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the input activation occurred) and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Initial value
• Average value
• Maximum value
• Minimum value
• Change

Section 11

Movement detector measures

ANY‑maze will score the following measures for a movement detector. Note that these measures are ONLY available for the apparatus as whole. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Count
• Time moving
• Latency to first movement

Section 12

Output switch measures

ANY‑maze will score the following measures for an output switch. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the output switch activation occurred), and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 13

Speaker measures

ANY‑maze will score the following measures for a speaker. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the speaker activation occurred), and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 14

Shocker measures

ANY‑maze will score the following measures for an shocker switch. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the shocker activation occurred), and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 15

Pellet dispenser measures

ANY‑maze will score the following measures for a pellet dispenser. Note that each measure is available for the apparatus as whole, and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of pellets dispensed
• Latency to first pellet dispensed

Section 16

Optogenetic laser controller measures

ANY‑maze will score the following measures for an optogenetic laser controller. Each measure is available for the apparatus as whole (i.e. irrespective of where the animal was when the laser controller activation occurred), and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 17

Syringe pump measures

ANY‑maze will score the following measures for a syringe pump. Note that these measures are ONLY available for the apparatus as whole. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Volume infused
• Volume withdrawn

Section 18

Light controller measures

ANY-maze will score the following measures for a light controller. Note that each measure is available for the apparatus as whole, and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 19

OPAD measures

ANY-maze will score the following measures for OPAD. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Temperature when contact broken
• Number of non-lick contacts
• Temperature of interest: time in contact
• Temperature of interest: number of times contact broken
• Temperature of interest: number of times contact made
• Temperature of interest: number of licks

Section 20

Virtual switch measures

ANY‑maze will score the following measures for a virtual switch. Note that each measure is available for the apparatus as whole, and also for each defined zone. For full details about each measure, including how it is calculated, see A detailed description of the ANY‑maze measures

• Number of activations
• Time active
• Latency to first activation
• Latency to first deactivation
• Distance travelled before 1st activation
• Distance travelled while active
• Longest activation
• Shortest activation
• Average activation duration
• Frequency of activations

Section 21

Procedure measures

Unlike almost all other measures in ANY‑maze, procedure measures do not update to reflect post-test changes in the procedure definition. Therefore, procedure measures are always based on the procedure that was in force when the test was performed.

Procedures don’t have any measures defined themselves, but you can create your own measures by using result variables. These are essentially numeric values that your procedure can set as it runs, and these values can then be included in ANY‑maze result analysis.

Once a procedure has been set up to use a result variable, a number of measures will be available for analysis. If the result variable is set up to be noted ‘only once, at the end of the test‘, then the following measure will be available for it:

• Value

If the result variable is set up to be noted ‘every time it is changed by the procedure‘ or ‘only when explicitly set‘, then the following measures will be available for it:

• Average value
• Maximum value
• Minimum value
• Sum of values
• Count of values
• List of values